Semen Volume: 1.5 mls (Normal Value: >1.5 mls)
Sperm Count (conc.): 34 million/ml (Normal Value: >15 million/ml)
Total Sperm Count: 51 million (Normal Value: >39 million)
Total Percent Motility: 29 % (Normal Value: >40 %)
Percent Rapid Progressive Motility: 40 % (Normal Value: >32 %)
Motile Sperm Count (conc.): 10 million/ml (Normal Value: n/a)
Total Motile Sperm: 15 million (Normal Value: n/a)
Round Cells: 0 million/ml (Normal Value: <5 million/ml)
Post Wash
Insemination Volume: 0.5 mls
Total Motile Sperm: 9 million
Wash Method: Density Gradient Centrifugation
Any idea why our total motile sperm went down from 15 million to 9 million post wash for our IUI? I was under the impression that the washing process just eliminated the sperm that weren't moving and kept all the motile ones.
Re: Question about sperm washing
You might have had
a premium wash:removes non-motile and bad quality
Or a swim-up technique and they keep the ones that 'swim-up'
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"Yes, some motile sperm can be lost during density gradient and washing step.First step is density gradient centrifugation that can separate dead sperm, debris or semen. After this, we take motile sperm in the bottom of the tube that passed through the gradient. Most of missing motile sperm is from this step. We only take about 80% of bottom portion in order to reduce any debris, dead sperm, or gradient solution.Then we do two times of sperm wash with fresh media to wash remaining gradient solution. During this step, most of sperm should be in the tube."
Me: 34, DH: 38 ~ TTC since 2014
IUI #1-3 (Nov 2015, Feb 2016, May 2016) = BFNs
IVF ER (July 2016) = 7 PGS normal embryos
FET #1 (Sept 2016) = BFP! DD born 5/30/17
FET #2 (April 2019) = BFN
FET #3 (July 2019) = BFP! DS born 3/27/20
My DH and I had the same thing. His original numbers looked good (a little low, but not concerning), but after the IUI wash we lost almost everything. It stinks, but they basically explained that what was left after washing was any that were good swimmers AND good looking.
---Trigger warning---
TTC since April 2013
Two cancelled IUIs in Aug and Sept '15 due to low motility/count
IVF round 1: January 2016
ER: February 17, 2016; 9 retrieved, 7 mature, 7 fertilized
Day 5 Blastocysts: 5 BB, 1 AB, all normal from PGS
FET date: May 11, '16; transferred 1 AB embryo
First beta: May 23, '16: 998!! Second: May 25, '16: 1648
EDD: January 27, 2017
Me: 34, DH: 38 ~ TTC since 2014
IUI #1-3 (Nov 2015, Feb 2016, May 2016) = BFNs
IVF ER (July 2016) = 7 PGS normal embryos
FET #1 (Sept 2016) = BFP! DD born 5/30/17
FET #2 (April 2019) = BFN
FET #3 (July 2019) = BFP! DS born 3/27/20
---Trigger warning---
TTC since April 2013
Two cancelled IUIs in Aug and Sept '15 due to low motility/count
IVF round 1: January 2016
ER: February 17, 2016; 9 retrieved, 7 mature, 7 fertilized
Day 5 Blastocysts: 5 BB, 1 AB, all normal from PGS
FET date: May 11, '16; transferred 1 AB embryo
First beta: May 23, '16: 998!! Second: May 25, '16: 1648
EDD: January 27, 2017