I always thought you just wash them once to prep, but the BG 4.0 I have that are bright colors (zinnia, moonbeam) have stickers on them recommending 6 washes separately before washing with lighter colors.
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Agree with PP - they only have to be washed once for absorbancy. However, BG recommends that you wash more so your colors don't run, although I haven't seen that as a common problem.
I always just wash microfiber once before using. They'll get more absorbent as they get washed more, but once has always been fine for me to go ahead and use.
Re: Pre-wash Kawaii's and BG 4.0