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During the day, I just pop them in the fridge and reuse. I have 4 sets of pump parts. One for my desk at work (it only took one time to forget them and have my shirt wet before I could get them) and one for the dishwasher, one for the drying rack and one for my bag- I am way overprepared. lol!Every night I take the parts completely apart. If I don't, they get milk buildup from the milkfat. It makes the pieces greasy feeling and mold will grow on them. I put them in the dishwasher basket but sometimes, I just use a bottle brush on the shields. I don't feel like I can get into the connectors good enough to get those clean with a bottle brush. I handwash the membranes and sanitize them about once a week. IN the dishwasher, I also do a sani rinse that is super heated to kill lingering bacteria and yeast. I have had thrush a few times and am militant about keeping the pump parts clean. When they are clean, I put them on the drying rack and put the membranes back on the yellow part when they are completely dry. You should def take them apart to clean- even the membranes. Milk can get in the crevices and mold.